Chemgenes Corporation, a DNA RNA Biotechnology company

Product Information: ((Dimethylamino-methylidene)amino)-3H-1,2,4-dithiazoline-3-thione (DDTT)3

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Available in bulk and formulated solution.
An efficient sulfurizing reagent with quantitative (greater than 99%) P/S conversion in oligonucleotide synthesis as compared to Beaucage reagent and has further advantage of greater stability in the formulated solutions.

A number of 1,2,4-dithiazolines are described in the literature1,2 and demonstrate high sulfurizing efficiency in oligonucleotide synthesis.

Our data has also shown superior sulfurization by DDTT reagent in solution phase. Unlike Beaucage reagent, DDTT did not yield any detectable level of phosphodiester type by-product.

Recommended formulations for the synthesis of posphorothioate containing oligonucleotides and sulfurizing in solution phase. > >

DDTT Concentration	Solvent formulation v/v	Oxidation time	Comments
0.05 M	Py/ACN - 3:7 Ph/THF - 2:8	2.5 min	Formulation used in oligonucleotide synthesis
0.1 M	PY Py/THF - 4:6	2 min	Formulation used in oligonucleotide synthesis
0.05 M	Acetonitrile	10 min	Formulation used in solution phase reaction